Resistome diversity in cattle and the environment decreases during beef productionNoelle R Noyes, Xiang Yang, Lyndsey M. Linke, Roberta J. Magnuson, Adam Dettenwanger, Shaun Cook, Ifigenia Geornaras, Dale R. Woerner , Sheryl P. Gow, Tim A. McAllister, Hua Yang, Jaime Ruiz, Kenneth L. Jones, Christina A. Boucher, Paul S. Morley, Keith E
Antimicrobial resistant determinants (ARDs) can be transmitted from livestock systems to humans through meat products or environmental effluents. However, the public health risk posed by these two routes is not well understood, particularly in non-pathogenic bacteria. We followed 1,741 commercial beef cattle from feedlot entry until resulting beef products were market-ready. We recorded antimicrobial drug exposures and utilized shotgun metagenomics to interrogate the resistome at critical points in beef production. Over 300 unique ARDs were identified. Resistome diversity decreased while cattle were in the feedlot, indicating selective pressure from antimicrobial drugs. ARDs were not identified in market-ready beef products, suggesting that interventions targeted at pathogens also reduce the risk of ARDs transmission to beef consumers. This report highlights the utility of metagenomics for assessing public health risks regarding antimicrobial resistance, and demonstrates that environmental exposure pathways may represent a more salient risk than the food supply chain.
Authors
Jaime Ruiz
As well as: Noelle R Noyes, Xiang Yang, Lyndsey M. Linke, Roberta J. Magnuson, Adam Dettenwanger, Shaun Cook, Ifigenia Geornaras, Dale R. Woerner , Sheryl P. Gow, Tim A. McAllister, Hua Yang, Kenneth L. Jones, Christina A. Boucher, Paul S. Morley, Keith E
Citation
Noelle R Noyes, Xiang Yang, Lyndsey M Linke, Roberta J Magnuson, Adam Dettenwanger, Shaun Cook, Ifigenia Geornaras, Dale E Woerner, Sheryl P Gow, Tim A McAllister, Hua Yang, Jaime Ruiz, Kenneth L Jones, Christina A Boucher, Paul S Morley, Keith E Belk (2016) Resistome diversity in cattle and the environment decreases during beef production eLife 5:e13195
Bibtex
@article {10.7554/eLife.13195,
article_type = {journal},
title = {Resistome diversity in cattle and the environment decreases during beef production},
author = {Noyes, Noelle R and Yang, Xiang and Linke, Lyndsey M and Magnuson, Roberta J and Dettenwanger, Adam and Cook, Shaun and Geornaras, Ifigenia and Woerner, Dale E and Gow, Sheryl P and McAllister, Tim A and Yang, Hua and Ruiz, Jaime and Jones, Kenneth L and Boucher, Christina A and Morley, Paul S and Belk, Keith E},
editor = {Cooper, Ben},
volume = 5,
year = 2016,
month = {mar},
pub_date = {2016-03-08},
pages = {e13195},
citation = {eLife 2016;5:e13195},
doi = {10.7554/eLife.13195},
url = {https://doi.org/10.7554/eLife.13195},
abstract = {Antimicrobial resistant determinants (ARDs) can be transmitted from livestock systems through meat products or environmental effluents. The public health risk posed by these two routes is not well understood, particularly in non-pathogenic bacteria. We collected pooled samples from 8 groups of 1741 commercial cattle as they moved through the process of beef production from feedlot entry through slaughter. We recorded antimicrobial drug exposures and interrogated the resistome at points in production when management procedures could potentially influence ARD abundance and/or transmission. Over 300 unique ARDs were identified. Resistome diversity decreased while cattle were in the feedlot, indicating selective pressure. ARDs were not identified in beef products, suggesting that slaughter interventions may reduce the risk of transmission of ARDs to beef consumers. This report highlights the utility and limitations of metagenomics for assessing public health risks regarding antimicrobial resistance, and demonstrates that environmental pathways may represent a greater risk than the food supply.},
keywords = {antimicrobial resistance, resistome, microbiome, agriculture, public health, feedlots},
journal = {eLife},
issn = {2050-084X},
publisher = {eLife Sciences Publications, Ltd},
}